Autologous plasma versus fetal calf serum as a supplement for the culture of neutrophils.

ABSTRACT: OBJECTIVE:Currently, the replacement of fetal calf serum (FCS) by a more suitable alternative is a sought aim in the field of tissue and cell culture research. Autologous plasma (AP) and especially autologous serum (AS) have been shown to be effective substitutes of FCS in culture media for some of the cell types. Nevertheless, there is no comparative data on the most appropriate supplement for cell media in neutrophil studies, it is now unclear whether AP have a relatively equal, superior or inferior performance to FCS in neutrophil cell culture. In the present study, human blood neutrophils were isolated and cultured in FCS- or AP-supplemented medium. After 12, 36 and 60 h of incubation, cell viability, oxidative burst and CD11b expression were determined by flow cytometry. RESULTS:Compa

SUBMITTER: Alipour R 

PROVIDER: S-EPMC6977324 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature