Single-cell genomics of uncultured bacteria reveals dietary fiber responders in the mouse gut microbiota.

ABSTRACT:

Background

The gut microbiota can have dramatic effects on host metabolism; however, current genomic strategies for uncultured bacteria have several limitations that hinder their ability to identify responders to metabolic changes in the microbiota. In this study, we describe a novel single-cell genomic sequencing technique that can identify metabolic responders at the species level without the need for reference genomes, and apply this method to identify bacterial responders to an inulin-based diet in the mouse gut microbiota.

Results

Inulin-feeding changed the mouse fecal microbiome composition to increase Bacteroides spp., resulting in the production of abundant succinate in the mouse intestine. Using our massively parallel single-cell genome sequencing technique, named S

SUBMITTER: Chijiiwa R 

PROVIDER: S-EPMC6977353 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature