Circulating microRNA expression profiling and bioinformatics analysis of patients with coronary artery disease by RNA sequencing.

ABSTRACT:

Background

MicroRNAs play a vital role in coronary artery disease. Abnormal expression of microRNAs has been found to be associated with the occurrence of CAD.

Methods

We identified significantly differentially expressed microRNAs in plasma between 40 patients with CAD and 10 controls with NCA using RNA sequencing. The differentially expressed microRNAs were analyzed for Gene Ontology (GO) enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment.

Results

Fifty cDNA libraries were constructed and sequenced, and a total of 1871.82 M raw reads were obtained, and 2135 microRNAs were found. Compared to the expressed microRNAs of NCA controls, 159 microRNAs were differentially expressed in CAD patients, including 119 upregulated microRNAs and 40 down

SUBMITTER: Zhong Z 

PROVIDER: S-EPMC6977390 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature