In vivo brain imaging with multimodal optical coherence microscopy in a mouse model of thromboembolic photochemical stroke.

ABSTRACT: We used a new multimodal imaging system that combines optical coherence microscopy and brightfield microscopy. Using this in vivo brain monitoring approach and cranial window implantation, we three-dimensionally visualized the vascular network during thrombosis, with high temporal (18 s) and spatial (axial, 2.5    μ m ; lateral, 2.2    μ m ) resolution. We used a modified mouse model of photochemical thromboembolic stroke in order to more accurately parallel human stroke. Specifically, we applied green laser illumination to focally occlude a branch of the middle cerebral artery. Despite the recanalization of the superficial arteries at 24 h after stroke, no blood flow was detected in the small vessels within deeper regions. Moreover, after 24 h of stroke progression, scattering si

SUBMITTER: Dolezyczek H 

PROVIDER: S-EPMC6977401 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature