Comparative analysis of cutaneous bacterial communities of farmed Rana dybowskii after gentamycin bath.

ABSTRACT:

Introduction

Pathogenic bacteria limit the success of Rana dybowskii breeding. Gentamicin is used to treat R. dybowskii disease. To understand the effects of gentamicin on the composition and structure of the cutaneous bacterial community of R. dybowskii, three groups (control, gentamicin and recovery) were established in this study.

Materials & methods

The V3-V4 hypervariable region of the 16S rRNA gene was analyzed in samples by high-throughput sequencing. Alpha diversity and beta diversity were evaluated to compare the cutaneous bacterial community diversity.

Results

A total of 1,159,668 valid sequences and 3,132 operational taxonomic units (OTUs) were obtained from these three experimental groups. The number of OTUs obtained in the control gro

SUBMITTER: Bie J 

PROVIDER: S-EPMC6977512 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature