Temperature-controlled electrospray ionization mass spectrometry as a tool to study collagen homo- and heterotrimers.

ABSTRACT: Collagen model peptides are useful for understanding the assembly and structure of collagen triple helices. The design of self-assembling heterotrimeric helices is particularly challenging and often affords mixtures of non-covalent assemblies that are difficult to characterize by conventional NMR and CD spectroscopic techniques. This can render a detailed understanding of the factors that control heterotrimer formation difficult and restrict rational design. Here, we present a novel method based on electrospray ionization mass spectrometry to investigate homo- and heterotrimeric collagen model peptides. Under native conditions, the high resolving power of mass spectrometry was used to access the stoichiometric composition of different triple helices in complex mixtures. A temperature-contr

SUBMITTER: Kohler M 

PROVIDER: S-EPMC6977553 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature