West Nile virus capsid protein inhibits autophagy by AMP-activated protein kinase degradation in neurological disease development.

ABSTRACT: West Nile virus (WNV) belongs to the Flaviviridae family and has emerged as a significant cause of viral encephalitis in birds and animals including humans. WNV replication directly induces neuronal injury, followed by neuronal cell death. We previously showed that accumulation of ubiquitinated protein aggregates was involved in neuronal cell death in the WNV-infected mouse brain. In this study, we attempted to elucidate the mechanisms of the accumulation of protein aggregates in the WNV-infected cells. To identify the viral factor inducing the accumulation of ubiquitinated proteins, intracellular accumulation of ubiquitinated proteins was examined in the cells expressing the viral protein. Expression of capsid (C) protein induced the accumulation, while mutations at residues L51 and A52 i

SUBMITTER: Kobayashi S 

PROVIDER: S-EPMC6977728 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature