Construction and evaluation of an efficient C-Jun siRNA to downregulate matrix metalloproteinase in human keratinocytes and fibroblasts under UV exposure.

ABSTRACT:

Background

C-Jun and EGFR have not been explored as targets via the mechanism of RNA silencing. Hence, this study designed an efficient C-Jun-h-825 small interfering RNA (siRNA) and investigated its effect on matrix metalloproteinase (MMP) and collagen expression in human keratinocytes exposed to UV radiation.

Methods

Five C-Jun siRNAs were designed and screened for their ability to downregulate C-Jun expression in human fibroblasts. These constructs were used to study changes in skin cancer-related protein expression. HaCaT cells were grouped into 5-carboxyfluorescien (FAM-labeled) C-Jun-h-825 siRNA + 2 hr prior irradiation; mock transfected + 2 hr prior irradiation; normal control; irradiation only for 2 hr; and Blank. Twenty-four hours posttransfection, mRNA and protein l

SUBMITTER: Xiao H 

PROVIDER: S-EPMC6978249 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature