Evaluation of screening platforms for virus-like particle production with the baculovirus expression vector system in insect cells.

ABSTRACT: Recombinant protein and virus-like particle (VLP) production based on the baculovirus expression vector system is fast, flexible, and offers high yields. Independent from the product, a multitude of parameters are screened during process development/optimisation. Early development acceleration is a key requirement for economic efficiency, and µ-scale bioreactor systems represent an attractive solution for high-throughput (HTP) experimentation. However, limited practical knowledge is available on the relevance and transferability of screening data to pilot scales and manufacturing. The main goal of the present study was to evaluate a HTP µ-bioreactor platform with respect to its aptitude as a screening platform mainly based on transferability of results to benchtop bioreactors representing

SUBMITTER: Strobl F 

PROVIDER: S-EPMC6978312 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature