SIMON: Simple methods for analyzing DNA methylation by targeted bisulfite next-generation sequencing.

ABSTRACT: DNA methylation in higher organisms has become an expanding field of study as it often involves the regulation of gene expression. Although Whole Genome Bisulfite Sequencing (WG-BS) based on next-generation sequencing (NGS) is the most versatile method, this is a costly technique that lacks in-depth analytic power. There are no conventional methods based on NGS that enable researchers to easily compare the level of DNA methylation from the practical number of samples handled in the laboratory. Although the targeted BS method based on Sanger sequencing is generally used in this case, it lacks in-depth analytic power. Therefore, we propose a new method that combines the high throughput analytic power of NGS and bioinformatics with the specificity and focus offered by PCR-amplification-based

SUBMITTER: Vial-Pradel S 

PROVIDER: S-EPMC6978500 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature