Ethanol Inhibits Aflatoxin B₁ Biosynthesis in Aspergillus flavus by Up-Regulating Oxidative Stress-Related Genes.

ABSTRACT: As the most carcinogenic, toxic, and economically costly mycotoxins, aflatoxin B₁ (AFB₁) is primarily biosynthesized by Aspergillus flavus and Aspergillus parasiticus. Aflatoxin biosynthesis is related to oxidative stress and functions as a second line of defense from excessive reactive oxygen species. Here, we find that ethanol can inhibit fungal growth and AFB₁ production by A. flavus in a dose-dependent manner. Then, the ethanol's molecular mechanism of action on AFB₁ biosynthesis was revealed using a comparative transcriptomic analysis. RNA-Seq data indicated that all the genes except for aflC in the aflatoxin gene cluster were down-regulated by 3.5% ethanol. The drastic repression of aflatoxin structural genes inc

SUBMITTER: Ren Y 

PROVIDER: S-EPMC6978751 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature