An Activity-Based Methionine Bioconjugation Approach To Developing Proximity-Activated Imaging Reporters.

ABSTRACT: Chemical probes that report on protein activity, rather than protein abundance, with spatial and temporal resolution can enable studies of their native function in biological contexts as well as provide opportunities for developing new types of biochemical reporters. Here we present a sensing platform, termed proximity-activated imaging reporter (PAIR), which combines activity-based methionine bioconjugation and antibody labeling with proximity-dependent oligonucleotide-based amplification to monitor dynamic changes of a given analyte in cells and animals through context-dependent methionine labeling of specific protein targets. We establish this PAIR method to develop sensors for imaging reactive oxygen species (ROS) and calcium ions through oxaziridine-directed labeling of reactive methi

SUBMITTER: Ohata J 

PROVIDER: S-EPMC6978837 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature