Development of a gp60-subtyping method for Cryptosporidium felis.

ABSTRACT:

Background

Feline cryptosporidiosis is an increasing problem, especially in catteries. In humans, close contact with cats could be a potential source of infection although the risk of contracting cryptosporidiosis caused by Cryptosporidium felis is considered to be relatively low. Sequencing of the 60-kDa glycoprotein gene is a commonly used tool for investigation of the genetic diversity and transmission dynamics of Cryptosporidium species. However, until now the sequence of gp60 from C. felis has not been available and genotyping has been limited to less discriminatory markers, such as 18S rRNA, COWP and HSP70.

Methods

We have identified the gp60 orthologue within the genome sequence of C. felis, and used the sequence to design a nested PCR for subtyping purposes. A total

SUBMITTER: Rojas-Lopez L 

PROVIDER: S-EPMC6979280 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature