Genome-wide CRISPR screening identifies new regulators of glycoprotein secretion.

ABSTRACT: Background: The fundamental process of protein secretion from eukaryotic cells has been well described for many years, yet gaps in our understanding of how this process is regulated remain. Methods: With the aim of identifying novel genes involved in the secretion of glycoproteins, we used a screening pipeline consisting of a pooled genome-wide CRISPR screen, followed by secondary siRNA screening of the hits to identify and validate several novel regulators of protein secretion. Results: We present approximately 50 novel genes not previously associated with protein secretion, many of which also had an effect on the structure of the Golgi apparatus. We further studied a small selection of hits to investigate their subcellular localisation. One of these, GPR161, is a nov

SUBMITTER: Popa S 

PROVIDER: S-EPMC6979480 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature