A microfluidic platform integrating pressure-driven and electroosmotic-driven flow with inline filters for affinity separations.

ABSTRACT: Pancreatic islets of Langerhans release glucagon to maintain blood glucose levels, and release of this peptide is dysregulated in diabetes mellitus. Although the importance of proper secretion of this peptide has been shown, no measurement of its release at the single islet level has been reported. In previous work, a non-competitive assay for glucagon was developed with a 6 pM limit of detection, low enough to measure from a single islet. To incorporate this method in an online assay, a microfluidic system with several distinct features was developed. To maintain appropriate flow rates in the presence of the high concentration of salt that was required for the assay, a piezo-actuated pressure transducer with in-line flow sensors was used to drive sample flow through 80 × 50 μm (width × de

SUBMITTER: Leng W 

PROVIDER: S-EPMC6980329 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature