Anchoring a dynamic in vitro model of human neuronal differentiation to key processes of early brain development in vivo.

ABSTRACT: We characterize temporal pathway dynamics of differentiation in an in vitro neurotoxicity model with the aim of informing design and interpretation of toxicological assays. Human neural progenitor cells (hNPCs) were cultured in differentiation conditions up to 21 days. Genes significantly changed through time were identified and grouped according to temporal dynamics. Quantitative pathway analysis identified gene ontology (GO) terms enriched among significantly changed genes and provided a temporal roadmap of pathway trends in vitro. Gene expression in hNPCs was compared with publicly available gene expression data from developing human brain tissue in vivo. Quantitative pathway analysis of significantly changed genes and targeted analysis of specific pathways of interest identified concor

SUBMITTER: Wegner SH 

PROVIDER: S-EPMC6980388 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature