Epilepsy in a melanocyte-lineage mTOR hyperactivation mouse model: A novel epilepsy model.

ABSTRACT:

Objective

To clarify the complex mechanism underlying epileptogeneis, a novel animal model was generated.

Methods

In our previous research, we have generated a melanocyte-lineage mTOR hyperactivation mouse model (Mitf-M-Cre Tsc2 KO mice; cKO mice) to investigate mTOR pathway in melanogenesis regulation, markedly reduced skin pigmentation was observed. Very unexpectedly, spontaneous recurrent epilepsy was also developed in this mouse model.

Results

Compared with control littermates, no change was found in either brain size or brain mass in cKO mice. Hematoxylin staining revealed no obvious aberrant histologic features in the whole brains of cKO mice. Histoimmunofluorescence staining and electron microscopy examination revealed markedly increased mTOR signaling and hype

SUBMITTER: Yang F 

PROVIDER: S-EPMC6980560 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature