Development of a microcolumn one-site immunometric assay for a protein biomarker: Analysis of alpha₁-acid glycoprotein.

ABSTRACT: A one-site immunometric assay based on affinity microcolumns was developed for the analysis of alpha₁-acid glycoprotein (AGP) as a model protein biomarker. In this assay, a sample containing AGP was incubated with an excess amount of a labeled binding agent, such as fluorescein-labeled anti-AGP antibodies or F_ab fragments. The excess binding agent was removed by passing this mixture through a microcolumn that contained an immobilized form of AGP, while the signal was measured for the binding agent-AGP complex in the non-retained fraction. Theoretical and practical factors were both considered in selecting the concentration of labeled binding agent, the incubation time of this agent with the sample, and the application conditions for this mixture onto the microcolumn.

SUBMITTER: Zhang C 

PROVIDER: S-EPMC6980775 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature