RASSF4 is required for skeletal muscle differentiation.

ABSTRACT: RASSF4, a member of the classical RASSF family of scaffold proteins, is associated with alveolar rhabdomyosarcoma, an aggressive pediatric cancer of muscle histogenesis. However, the role of RASSF4 in normal myogenesis is unknown. We demonstrate here that RASSF4 is necessary for early in vitro myogenesis. Using primary human myoblasts, we show that RASSF4 expression is dramatically increased during in vitro myogenic differentiation, and conversely that RASSF4-deficient myoblasts cannot differentiate, potentially because of a lack of upregulation of myogenin. In microscopy studies, we show that RASSF4 protein co-localizes with proteins of the myogenic microtubule-organizing center (MTOC) both before and after myogenic differentiation. RASSF4-deficient cells subject to differentiation condit

SUBMITTER: Lin YT 

PROVIDER: S-EPMC6980882 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature