Transendothelial Insulin Transport is Impaired in Skeletal Muscle Capillaries of Obese Male Mice.

ABSTRACT:

Objective

The continuous endothelium of skeletal muscle (SkM) capillaries regulates insulin's access to skeletal myocytes. Whether impaired transendothelial insulin transport (EIT) contributes to SkM insulin resistance (IR), however, is unknown.

Methods

Male and female C57/Bl6 mice were fed either chow or a high-fat diet for 16 weeks. Intravital microscopy was used to measure EIT in SkM capillaries, electron microscopy to assess endothelial ultrastructure, and glucose tracers to measure indices of glucose metabolism.

Results

Diet-induced obesity (DIO) male mice were found to have a ~15% reduction in EIT compared with lean mice. Impaired EIT was associated with a 45% reduction in endothelial vesicles. Despite impaired EIT, hyperinsulinemia sustained delivery of insulin

SUBMITTER: Williams IM 

PROVIDER: S-EPMC6980999 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature