T follicular helper and memory cell responses and the mTOR pathway in murine heart transplantation.

ABSTRACT:

Background

The mammalian target of rapamycin (mTOR) inhibitors are valuable immunosuppressants in clinical transplantation; however, the mTOR regulation of allogeneic T-cell responses is not fully understood yet. Therefore, the objective of this study is to investigate the effects of T-cell-specific mTOR deletion on the allogeneic T-cell responses and heart transplant survival.

Methods

BALB/c heart allografts, with or without BALB/c skin sensitization, were transplanted in the wild-type C57BL/6, Mtor^fl/flCd4-Cre, Stat3^fl/flCd4-Cre, and Mtor^fl/flStat3^fl/flCd4-Cre mice. Graft survival and histology, as well as T-cell frequencies and phenotypes, were evaluated after transplantation.

Results

In the absence of donor skin sensitiz

SUBMITTER: Xie A 

PROVIDER: S-EPMC6981023 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature