CD4⁺ T Cell-Derived NGAL Modifies the Outcome of Ischemic Acute Kidney Injury.

ABSTRACT: CD4⁺ T cells mediate the pathogenesis of ischemic and nephrotoxic acute kidney injury (AKI). However, the underlying mechanisms of CD4⁺ T cell-mediated pathogenesis are largely unknown. We therefore conducted unbiased RNA-sequencing to discover novel mechanistic pathways of kidney CD4⁺ T cells after ischemia compared with normal mouse kidney. Unexpectedly, the lipocalin-2 (Lcn2) gene, which encodes neutrophil gelatinase-associated lipocalin (NGAL) had the highest fold increase (∼60). The NGAL increase in CD4⁺ T cells during AKI was confirmed at the mRNA level with quantitative real-time PCR and at the protein level with ELISA. NGAL is a potential biomarker for the early detection of AKI and has multiple potential biological functions. Howe

SUBMITTER: Lee SA 

PROVIDER: S-EPMC6981061 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature