Nanodisc technology facilitates identification of monoclonal antibodies targeting multi-pass membrane proteins.

ABSTRACT: Multi-pass membrane proteins are important targets of biologic medicines. Given the inherent difficulties in working with membrane proteins, we sought to investigate the utility of membrane scaffold protein nanodiscs as a means of solubilizing membrane proteins to aid antibody discovery. Using a model multi-pass membrane protein, we demonstrate how incorporation of a multi-pass membrane protein into nanodiscs can be used in flow cytometry to identify antigen-specific hybridoma. The use of target protein-loaded nanodiscs to sort individual hybridoma early in the screening process can reduce the time required to identify antibodies against multi-pass membrane proteins.

SUBMITTER: Gardill B 

PROVIDER: S-EPMC6981118 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature