DNA hypomethylation during MSC chondrogenesis occurs predominantly at enhancer regions.

ABSTRACT: Regulation of transcription occurs in a cell type specific manner orchestrated by epigenetic mechanisms including DNA methylation. Methylation changes may also play a key role in lineage specification during stem cell differentiation. To further our understanding of epigenetic regulation in chondrocytes we characterised the DNA methylation changes during chondrogenesis of mesenchymal stem cells (MSCs) by Infinium 450 K methylation array. Significant DNA hypomethylation was identified during chondrogenic differentiation including changes at many key cartilage gene loci. Integration with chondrogenesis gene expression data revealed an enrichment of significant CpGs in upregulated genes, while characterisation of significant CpG loci indicated their predominant localisation to enhancer region

SUBMITTER: Barter MJ 

PROVIDER: S-EPMC6981252 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature