Differences in Stability of Viral and Viral-Cellular Fusion Transcripts in HPV-Induced Cervical Cancers.

ABSTRACT: HPV-DNA integration results in dysregulation of viral oncogene expression. Because viral-cellular fusion transcripts inherently lack the viral AU-rich elements of the 3'UTR, they are considered to be more stable than episome-derived transcripts. The aim of this study is to provide formal proof for this assumption by comparing the stability of viral early transcripts derived from episomal and integrated HPV16 DNA, respectively. Full-length cDNA of three fusion transcripts comprising viral and cellular sequences in sense orientation were amplified and cloned into the adeno-viral-vector pAd/CMV/V5-DEST. The most abundant HPV16 oncogene transcript E6*I_-E7_-E1_vE4-E5 with and without 3'UTR, served as reference and control, respectively. Human primary keratinocytes

SUBMITTER: Ehrig F 

PROVIDER: S-EPMC6981427 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature