In Situ Proteolysis Condition-Induced Crystallization of the XcpVWX Complex in Different Lattices.

ABSTRACT: Although prevalent in the determination of protein structures; crystallography always has the bottleneck of obtaining high-quality protein crystals for characterizing a wide range of proteins; especially large protein complexes. Stable fragments or domains of proteins are more readily to crystallize; which prompts the use of in situ proteolysis to remove flexible or unstable structures for improving crystallization and crystal quality. In this work; we investigated the effects of in situ proteolysis by chymotrypsin on the crystallization of the XcpVWX complex from the Type II secretion system of Pseudomonas aeruginosa. Different proteolysis conditions were found to result in two distinct lattices in the same crystallization solution. With a shorter chymotrypsin digestion at a lower

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC6981927 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature