Salivary Microbiome and Cigarette Smoking: A First of Its Kind Investigation in Jordan.

ABSTRACT: There is accumulating evidence in the biomedical literature suggesting the role of smoking in increasing the risk of oral diseases including some oral cancers. Smoking alters microbial attributes of the oral cavity by decreasing the commensal microbial population and increasing the pathogenic microbes. This study aims to investigate the shift in the salivary microbiota between smokers and non-smokers in Jordan. Our methods relied on high-throughput next-generation sequencing (NGS) experiments for V3-V4 hypervariable regions of the 16S rRNA gene, followed by comprehensive bioinformatics analysis including advanced multidimensional data visualization methods and statistical analysis approaches. Six genera-Streptococcus, Prevotella, Vellionella, Rothia, Neisseria, and Haemophilus-predominated

SUBMITTER: Al-Zyoud W 

PROVIDER: S-EPMC6982339 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature