The vaginal metabolome and microbiota of cervical HPV-positive and HPV-negative women: a cross-sectional analysis.

ABSTRACT:

Objective

Characterise the vaginal metabolome of cervical HPV-infected and uninfected women.

Design

Cross-sectional.

Setting

The Center for Health Behavior Research at the University of Maryland School of Public Health.

Sample

Thirty-nine participants, 13 categorised as HPV-negative and 26 as HPV-positive (any genotype; HPV⁺ ), 14 of whom were positive with at least one high-risk HPV strain (hrHPV).

Method

Self-collected mid-vaginal swabs were profiled for bacterial composition by 16S rRNA gene amplicon sequencing, metabolites by both gas and liquid chromatography mass spectrometry, and 37 types of HPV DNA.

Main outcome measures

Metabolite abundances.

Results

Vaginal microbiota clustered into Community State Type (CST) I (Lactoba

SUBMITTER: Borgogna JC 

PROVIDER: S-EPMC6982399 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature