Reduced expression of the nob8 gene does not normalize the distribution or function of mGluR6 in the mouse retina.

ABSTRACT: Purpose:The Grm6nob8 mouse carries a missense mutation in the Grm6 gene (p.Met66Leu), and exhibits a reduced b-wave of the electroretinogram (ERG), abnormal localization of metabotropic glutamate receptor 6 (mGluR6) to the depolarizing bipolar cell (DBC) soma, and a reduced level of mGluR6 at the DBC dendritic tips. Although the underlying mechanism remains unknown, one possible explanation is that DBCs cannot efficiently traffic the mutant mGluR6. In that scenario, reducing the total amount of mutant mGluR6 protein might normalize localization, and thus, improve the ERG phenotype as well. The second purpose of this study was to determine whether the abnormal cellular distribution of mutant mGluR6 in Grm6nob8 retinas might induce late onset DBC degeneration. Methods:We crossed Grm6nob8 ani

SUBMITTER: Kinoshita J 

PROVIDER: S-EPMC6982428 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature