Imaging the distribution of iron oxide nanoparticles in hypothermic perfused tissues.

ABSTRACT:

Purpose

Herein, we evaluate the use of MRI as a tool for assessing iron oxide nanoparticle (IONP) distribution within IONP perfused organs and vascularized composite allografts (VCAs) (i.e., hindlimbs) prepared for cryopreservation.

Methods

Magnetic resonance imaging was performed on room-temperature organs and VCAs perfused with IONPs and were assessed at 9.4 T. Quantitative T₁ mapping and T2∗ -weighted images were acquired using sweep imaging with Fourier transformation and gradient-echo sequences, respectively. Verification of IONP localization was performed through histological assessment and microcomputer tomography.

Results

Quantitative imaging was achieved for organs and VCAs perfused with up to 642 mM_Fe (36 mg_Fe /mL), which is

SUBMITTER: Ring HL 

PROVIDER: S-EPMC6982566 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature