Quantitative classification of chromatin dynamics reveals regulators of intestinal stem cell differentiation.

ABSTRACT: Intestinal stem cell (ISC) plasticity is thought to be regulated by broadly permissive chromatin shared between ISCs and their progeny. Here, we have used a Sox9^EGFP reporter to examine chromatin across ISC differentiation. We find that open chromatin regions (OCRs) can be defined as broadly permissive or dynamic in a locus-specific manner, with dynamic OCRs found primarily in loci consistent with distal enhancers. By integrating gene expression with chromatin accessibility at transcription factor (TF) motifs in the context of Sox9^EGFP populations, we classify broadly permissive and dynamic chromatin relative to TF usage. These analyses identify known and potential regulators of ISC differentiation via association with dynamic changes in chromatin. Consistent with com

SUBMITTER: Raab JR 

PROVIDER: S-EPMC6983707 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature