Single cell sequencing of radial glia progeny reveals the diversity of newborn neurons in the adult zebrafish brain.

ABSTRACT: Zebrafish display widespread and pronounced adult neurogenesis, which is fundamental for their regeneration capability after central nervous system injury. However, the cellular identity and the biological properties of adult newborn neurons are elusive for most brain areas. Here, we have used short-term lineage tracing of radial glia progeny to prospectively isolate newborn neurons from the her4.1⁺ radial glia lineage in the homeostatic adult forebrain. Transcriptome analysis of radial glia, newborn neurons and mature neurons using single cell sequencing identified distinct transcriptional profiles, including novel markers for each population. Specifically, we detected two separate newborn neuron types, which showed diversity of cell fate commitment and location. Further analys

SUBMITTER: Lange C 

PROVIDER: S-EPMC6983714 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature