Genet assignment and population structure analysis in a clonal forest-floor herb, Cardamine leucantha, using RAD-seq.

ABSTRACT: To study the genetic structure of clonal plant populations, genotyping and genet detection using genetic markers are necessary to assign ramets to corresponding genets. Assignment is difficult as it involves setting a robust threshold of genetic distance for genet distinction as neighbouring genets in a plant population are often genetically related. Here, we used restriction site-associated DNA sequencing (RAD-seq) for a rhizomatous clonal herb, Cardamine leucantha [Brassicaceae] to accurately determine genet structure in a natural population. We determined a draft genome sequence of this species for the first time, which resulted in 66 617 scaffolds with N₅₀ = 6086 bp and an estimated genome size of approximately 253 Mbp. Using genetic distances based on the RAD-seq ana

SUBMITTER: Tsujimoto M 

PROVIDER: S-EPMC6983914 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature