Discovery of novel [FeFe]-hydrogenases for biocatalytic H₂-production.

ABSTRACT: A new screening method for [FeFe]-hydrogenases is described, circumventing the need for specialized expression conditions as well as protein purification for initial characterization. [FeFe]-hydrogenases catalyze the formation and oxidation of molecular hydrogen at rates exceeding 10³ s^-1, making them highly promising for biotechnological applications. However, the discovery of novel [FeFe]-hydrogenases is slow due to their oxygen sensitivity and dependency on a structurally unique cofactor, complicating protein expression and purification. Consequently, only a very limited number have been characterized, hampering their implementation. With the purpose of increasing the throughput of [FeFe]-hydrogenase discovery, we have developed a screening method that allows for r

SUBMITTER: Land H 

PROVIDER: S-EPMC6984386 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature