Cell quality control mechanisms maintain stemness and differentiation potential of P19 embryonic carcinoma cells.

ABSTRACT: Given the relatively long life of stem cells (SCs), efficient mechanisms of quality control to balance cell survival and resistance to external and internal stress are required. Our objective was to test the relevance of cell quality control mechanisms for SCs maintenance, differentiation and resistance to cell death. We compared cell quality control in P19 stem cells (P19SCs) before and after differentiation (P19dCs). Differentiation of P19SCs resulted in alterations in parameters involved in cell survival and protein homeostasis, including the redox system, cardiolipin and lipid profiles, unfolded protein response, ubiquitin-proteasome and lysosomal systems, and signaling pathways controlling cell growth. In addition, P19SCs pluripotency was correlated with stronger antioxidant protectio

SUBMITTER: Magalhaes-Novais S 

PROVIDER: S-EPMC6984605 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature