The C-terminal region of ATG101 bridges ULK1 and PtdIns3K complex in autophagy initiation.

ABSTRACT: The initiation of macroautophagy/autophagy is tightly regulated by the upstream ULK1 kinase complex, which affects many downstream factors including the PtdIns3K complex. The phosphorylation of the right position at the right time on downstream molecules is governed by proper complex formation. One component of the ULK1 complex, ATG101, known as an accessory protein, is a stabilizer of ATG13 in cells. The WF finger region of ATG101 plays an important role in the recruitment of WIPI1 (WD repeat domain, phosphoinositide interacting protein 1) and ZFYVE1 (zinc finger FYVE-type containing 1). Here, we report that the C-terminal region identified in the structure of the human ATG101-ATG13^HORMA complex is responsible for the binding of the PtdIns3K complex. This region adopts a β-stra

SUBMITTER: Kim BW 

PROVIDER: S-EPMC6984762 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature