Differential Gene Expression Patterns Between Apical and Basal Inner Hair Cells Revealed by RNA-Seq.

ABSTRACT: Tonotopic differences in the structure and physiological function, e.g., synapse number, membrane properties, Ca²⁺ channels, Ca²⁺ dependence of exocytosis and vesicle pool replenishment of inner hair cells (IHCs) along the longitudinal cochlear axis have recently been discovered, suggesting different gene expression patterns of IHCs. To determine whether IHCs present different gene expression patterns along the longitudinal cochlear axis, apical and basal IHCs were collected separately using the suction pipette technique from adult mouse cochleae for RNA-seq and genome-wide transcriptome analysis. We found 689 annotated genes showed more than 2-fold increase in expression. Interestingly, 93.4% of the differentially expressed genes (DEGs) was upregulated in apical IHCs

SUBMITTER: Tang F 

PROVIDER: S-EPMC6985465 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature