Rbm14 maintains the integrity of genomic DNA during early mouse embryogenesis via mediating alternative splicing.

ABSTRACT:

Objective

In this study, we generated an Rbm14 knockout mouse model to explore its functions during early mouse embryogenesis.

Materials and methods

The Rbm14 knockout mouse model was generated by a combination of clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 and microinjection techniques. The developmental defects of the knockout embryos were characterized by histological analyses. The accumulation of DNA damage in mouse embryonic stem cells (ESCs) was detected by γH2AX staining and comet assay. The altered mRNA splicing of DNA damage response (DDR)-related genes was detected by RNA-Seq analysis and confirmed by semi-quantitative PCR. The interaction of RBM14 with alternative splicing-related genes was detected by immunoprecipitation-mass spectra (

SUBMITTER: Li J 

PROVIDER: S-EPMC6985654 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature