SHP2 deficiency promotes Staphylococcus aureus pneumonia following influenza infection.

ABSTRACT:

Objectives

Secondary bacterial pneumonia is common following influenza infection. However, it remains unclear about the underlying molecular mechanisms.

Materials and methods

We established a mouse model of post-influenza S aureus pneumonia using conditional Shp2 knockout mice (LysM^Cre/+ :Shp2^flox/flox ). The survival, bacterial clearance, pulmonary histology, phenotype of macrophages, and expression of type I interferons and chemokines were assessed between SHP2 deletion and control mice (Shp2^flox/flox ). We infused additional KC and MIP-2 to examine the reconstitution of antibacterial immune response in LysM^Cre/+ :Shp2^flox/flox mice. The effect of SHP2 on signal molecules including MAPKs (JNK, p38 and Erk1/2), NF-κB p6

SUBMITTER: Ouyang W 

PROVIDER: S-EPMC6985656 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature