Inhibition Activity of Avibactam against Nocardia farcinica β-Lactamase FAR_IFM10152.

ABSTRACT: Nocardia farcinica, one of the most frequent pathogenic species responsible for nocardiosis, is characterized by frequent brain involvement and resistance to β-lactams mediated by a class A β-lactamase. Kinetic parameters for hydrolysis of various β-lactams by FAR_IFM10152 from strain IFM 10152 were determined by spectrophotometry revealing a high catalytic activity (k_cat/K_m ) for amoxicillin, aztreonam, and nitrocefin. For cephems, k_cat/K_m was lower but remained greater than 10⁴ M^-1 s^-1 A low catalytic activity was observed for meropenem, imipenem, and ceftazidime hydrolysis. FAR_IFM10152 inhibition by avibactam and clavulanate was compared using nitrocefin a

SUBMITTER: Lebeaux D 

PROVIDER: S-EPMC6985717 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature