Sterol structure dependence of insulin receptor and insulin-like growth factor 1 receptor activation.

ABSTRACT: The plasma membrane is a dynamic environment with a complex composition of lipids, proteins, and cholesterol. Areas enriched in cholesterol and sphingolipids are believed to form lipid rafts, domains of highly ordered lipids. The unique physical properties of these domains have been proposed to influence many cellular processes. Here, we demonstrate that the activation of insulin receptor (IR) and insulin-like growth factor 1 receptor (IGF1R) depends critically on the structures of membrane sterols. IR and IGF1R autophosphorylation in vivo was inhibited by cholesterol depletion, and autophosphorylation was restored by the replacement with exogenous cholesterol. We next screened a variety of sterols for effects on IR activation. The ability of sterols to support IR autophosphorylation was s

SUBMITTER: Delle Bovi RJ 

PROVIDER: S-EPMC6985913 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature