Studying the urine microbiome in superficial bladder cancer: samples obtained by midstream voiding versus cystoscopy.

ABSTRACT:

Background

Preliminary data suggest that the urinary microbiome may play a role in bladder cancer. Information regarding the most suitable method of collecting urine specimens is needed for the large population studies needed to address this. To compare microbiome metrics resulting from 16S ribosomal RNA gene sequencing between midstream, voided specimens and those obtained at cystoscopy.

Methods

Adults, with a history of superficial urothelial cell carcinoma (non-muscle invasive bladder cancer) being followed with periodic surveillance cystoscopy had a urine sample collected by a mid-stream, voided technique and then from the bladder at cystoscopy. Urine samples underwent 16S ribosomal RNA gene sequencing on the Illumina MiSeq platform.

Results

22 subjects (8 female,

SUBMITTER: Hourigan SK 

PROVIDER: S-EPMC6986141 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature