GLI transcriptional repression regulates tissue-specific enhancer activity in response to Hedgehog signaling.

ABSTRACT: Transcriptional repression needs to be rapidly reversible during embryonic development. This extends to the Hedgehog pathway, which primarily serves to counter GLI repression by processing GLI proteins into transcriptional activators. In investigating the mechanisms underlying GLI repression, we find that a subset of GLI binding regions, termed HH-responsive enhancers, specifically loses acetylation in the absence of HH signaling. These regions are highly enriched around HH target genes and primarily drive HH-specific transcriptional activity in the mouse limb bud. They also retain H3K27ac enrichment in limb buds devoid of GLI activator and repressor, indicating that their activity is primarily regulated by GLI repression. Furthermore, the Polycomb repression complex is not active at most

SUBMITTER: Lex RK 

PROVIDER: S-EPMC6986877 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature