LongAxis: A MATLAB-based program for 3D quantitative analysis of epithelial cell shape and orientation.

ABSTRACT: Epithelial morphogenesis, a fundamental aspect of development, generates 3-dimensional tissue structures crucial for organ function. Underlying morphogenetic mechanisms are, in many cases, poorly understood, but mutations that perturb organ development can affect epithelial cell shape and orientation - difficult features to quantify in three dimensions. The basic structure of the eye is established via epithelial morphogenesis: in the embryonic optic cup, the retinal progenitor epithelium enwraps the lens. We previously found that loss of the extracellular matrix protein laminin-alpha1 (lama1) led to mislocalization of apical polarity markers and apparent misorientation of retinal progenitors. We sought to visualize and quantify this phenotype, and determine whether loss of the apical pola

SUBMITTER: Carney KR 

PROVIDER: S-EPMC6987011 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature