Building de novo reference genome assemblies of complex eukaryotic microorganisms from single nuclei.

ABSTRACT: The advent of novel sequencing techniques has unraveled a tremendous diversity on Earth. Genomic data allow us to understand ecology and function of organisms that we would not otherwise know existed. However, major methodological challenges remain, in particular for multicellular organisms with large genomes. Arbuscular mycorrhizal (AM) fungi are important plant symbionts with cryptic and complex multicellular life cycles, thus representing a suitable model system for method development. Here, we report a novel method for large scale, unbiased nuclear sorting, sequencing, and de novo assembling of AM fungal genomes. After comparative analyses of three assembly workflows we discuss how sequence data from single nuclei can best be used for different downstream analyses such as phylogenomics

SUBMITTER: Montoliu-Nerin M 

PROVIDER: S-EPMC6987183 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature