Studies on expression levels of pil Q and fli P genes during bio-electrogenic process in Kluyvera georgiana MCC 3673.

ABSTRACT: The bacterium Kluyvera georgiana MCC 3673 transfers electrons directly to the electrode for bio-electricity generation in microbial fuel cell (MFC). This could be due to the formation of biofilm on the surface of electrode or with through the extracellular appendages, or both. The role of extracellular appendages pili and flagella in exo-electron transfer mechanism was investigated. The expression level of the genes fli P and pil Q for pili and flagella, respectively, in K. georgiana MCC 3673 was compared in MFC and in shake flask. The transcript analysis was done by qRT-PCR at different times and conditions. The expression level of pil Q transcript in K. georgiana MCC 3673 showed over twofold higher expression during bio-electrogenic process, comp

SUBMITTER: Thapa BS 

PROVIDER: S-EPMC6987287 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature