Cohesin Disrupts Polycomb-Dependent Chromosome Interactions in Embryonic Stem Cells.

ABSTRACT: How chromosome organization is related to genome function remains poorly understood. Cohesin, loop extrusion, and CCCTC-binding factor (CTCF) have been proposed to create topologically associating domains (TADs) to regulate gene expression. Here, we examine chromosome conformation in embryonic stem cells lacking cohesin and find, as in other cell types, that cohesin is required to create TADs and regulate A/B compartmentalization. However, in the absence of cohesin, we identify a series of long-range chromosomal interactions that persist. These correspond to regions of the genome occupied by the polycomb repressive system and are dependent on PRC1. Importantly, we discover that cohesin counteracts these polycomb-dependent interactions, but not interactions between super-enhancers. This dis

SUBMITTER: Rhodes JDP 

PROVIDER: S-EPMC6988126 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature