Human CardioChimeras: Creation of a Novel "Next-Generation" Cardiac Cell.

ABSTRACT: Background CardioChimeras produced by fusion of murine c-kit⁺ cardiac interstitial cells with mesenchymal stem cells promote superior structural and functional recovery in a mouse model of myocardial infarction compared with either precursor cell alone or in combination. Creation of human CardioChimeras (hCCs) represents the next step in translational development of this novel cell type, but new challenges arise when working with c-kit⁺ cardiac interstitial cells isolated and expanded from human heart tissue samples. The objective of the study was to establish a reliable cell fusion protocol for consistent optimized creation of hCCs and characterize fundamental hCC properties. Methods and Results Cell fusion was induced by incubating human c-kit⁺ cardiac in

SUBMITTER: Firouzi F 

PROVIDER: S-EPMC6988174 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature