Development and characterization of Escherichia coli triple reporter strains for investigation of population heterogeneity in bioprocesses.

ABSTRACT: BACKGROUND:Today there is an increasing demand for high yielding robust and cost efficient biotechnological production processes. Although cells in these processes originate from isogenic cultures, heterogeneity induced by intrinsic and extrinsic influences is omnipresent. To increase understanding of this mechanistically poorly understood phenomenon, advanced tools that provide insights into single cell physiology are needed. RESULTS:Two Escherichia coli triple reporter strains have been designed based on the industrially relevant production host E. coli BL21(DE3) and a modified version thereof, E. coli T7E2. The strains carry three different fluorescence proteins chromosomally integrated. Single cell growth is followed with EmeraldGFP (EmGFP)-expression together with the ribosomal promot

SUBMITTER: Heins AL 

PROVIDER: S-EPMC6988206 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature