Fluorescent amplification for next generation sequencing (FA-NGS) library preparation.

ABSTRACT: BACKGROUND:Next generation sequencing (NGS) has become a universal practice in modern molecular biology. As the throughput of sequencing experiments increases, the preparation of conventional multiplexed libraries becomes more labor intensive. Conventional library preparation typically requires quality control (QC) testing for individual libraries such as amplification success evaluation and quantification, none of which occur until the end of the library preparation process. RESULTS:In this study, we address the need for a more streamlined high-throughput NGS workflow by tethering real-time quantitative PCR (qPCR) to conventional workflows to save time and implement single tube and single reagent QC. We modified two distinct library preparation workflows by replacing PCR and quantificatio

SUBMITTER: Chiniquy J 

PROVIDER: S-EPMC6988211 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature